Identifiant pérenne de la notice : 21036484X
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Note publique d'information : Caspases, Paracaspases, and Metacaspacses: Methods and Protocols is a collection
of laboratory protocols covering current methods that are employed to measure and
detect activities of these proteases in diverse biological systems, ranging from unicellular
organisms to mammals. Broken into two parts, the first part focuses on methods to
measure, detect, and inhibit activation and activity of a subset of or specific caspases
in vitro and in several model systems and organisms, primarily in the context of programmed
cell death. The second part of the book provides experimental protocols for purification
and in vitro and in vivo analysis of yeast, protozoan and plant metacaspases, as well
as of a human paracaspase MALT1. Written in the highly successful Methods in Molecular
Biology series format, the chapters include the kind of detailed description and implementation
advice that is crucial for getting optimal results in the laboratory. Authoritative
and practical, Caspases, Paracaspases, and Metacaspacses: Methods and Protocols seeks
to aid scientists easy-to-follow techniques