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Note publique d'information : In this second edition methods are described to measure the synthesis of lipids such
as the phosphoinositides, ceramides and sphingomyelin, as well as techniques to molecularly
characterize the various kinases and phosphatases that regulate the intracellular
metabolism of these lipids. Lipid Signaling Protocols, Second Edition guides readers
through detailed experimental protocols, which are complimented by review chapters
that highlight the technical considerations, challenges and potential pitfalls associated
with using these laboratory-based approaches. Written for the Methods in Molecular
Biology series, chapters include introductions to their respective topics, lists of
the necessary materials and reagents, step-by-step, readily reproducible laboratory
protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative
and practical, Lipid Signaling Protocols, Second Edition aims to ensure successful
results in the further study of this vital field.
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